Troponin I Type 3 (cardiac) Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Troponin I Type 3 (cardiac) Antibody [NBP2-33463] - Staining of human cell line HEK 293 shows localization to cytosol & vesicles.

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-33463] - Staining of human prostate shows low expression as expected.

Immunohistochemistry: Troponin I Type 3 (cardiac) Antibody [NBP2-33463] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-33463] - Staining in human heart muscle and prostate tissues using anti-TNNI3 antibody. Corresponding TNNI3 RNA-seq data are presented for the same tissues.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Troponin I Type 3 (cardiac) Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Troponin I Type 3 (cardiac) Antibody - BSA Free (NBP2-33463) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: PIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQ
• Predicted Species: Mouse (90%), Rat (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNNI3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Troponin I Type 3 (cardiac) Protein (NBP2-33463PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Troponin I Type 3 (cardiac) Antibody - BSA Free

• Cardiac troponin I
• CMD1FF
• CMD2A
• CMH7MGC116817
• CTnI
• familial hypertrophic cardiomyopathy 7
• RCM1
• TNNC1
• TNNC1troponin I, cardiac
• TNNI3,
• troponin I type 3 (cardiac)
• troponin I, cardiac muscle
• Troponin I/TNNI3

Background

Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNNI3
• Uniprot:
  • P19429()