Troponin I Type 3 (cardiac) [p Ser150] Antibody

Images

 
Western Blot: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Western blot of mouse heart lysate showing specific immunolabeling of ~25k cTnI protein phosphorylated at Ser150 (control). Phosphospecificity ...read more
Immunofluorescence: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Fixed adult mouse cardiomyocyte were stained with anti-Pim-1 and anti-cTnI antibodies and then processed for fluorescentstaining ...read more
Immunoprecipitation: Troponin I Type 3 (cardiac) [p Ser150] Antibody [NBP2-29526] - Adult mouse heart tissue samples were extracted and immunoprecipitated with either anti-Pim-1 antibody or control IgG and then analyzed ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Hu, PmSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Troponin I Type 3 (cardiac) [p Ser150] Antibody Summary

Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 conjugated to KLH. Accession # P48787
Modification
p Ser150
Specificity
Specific for endogenous levels of the ~25 kDa Troponin I Type 3 (cardiac) protein phosphorylated at Ser150. Immunolabeling is greatly decreased with lambda-phosphatase treatment.
Preparation
Method
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Predicted Species
Human (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TNNI3
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1:1000
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-29526 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Antigen Affinity-purified

Alternate Names for Troponin I Type 3 (cardiac) [p Ser150] Antibody

  • Cardiac troponin I
  • CMD1FF
  • CMD2A
  • CMH7MGC116817
  • CTnI
  • familial hypertrophic cardiomyopathy 7
  • RCM1
  • TNNC1
  • TNNC1troponin I, cardiac
  • TNNI3,
  • troponin I type 3 (cardiac)
  • troponin I, cardiac muscle
  • Troponin I/TNNI3

Background

Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 (Noland et al, 1995) and is phosphorylated by AMPK at Ser23 and Ser150 (Solis et al, 2011). Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser150 over Ser23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI (Solis et al., 2011).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Troponin I Type 3 (cardiac) Antibody (NBP2-29526)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for Troponin I Type 3 (cardiac) Antibody (NBP2-29526) (0)

There are no reviews for Troponin I Type 3 (cardiac) Antibody (NBP2-29526). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Troponin I Type 3 (cardiac) Antibody (NBP2-29526) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Troponin I Type 3 (cardiac) Products

Research Areas for Troponin I Type 3 (cardiac) Antibody (NBP2-29526)

Find related products by research area.

Blogs on Troponin I Type 3 (cardiac).

Troponin I Type 3 (TnI, cardiac)
Troponin I is part of a heteromeric thin filament regulatory complex that governs skeletal and cardiac muscle contraction. Troponin I is composed of three subunits: troponin I (TnI), troponin T (TnT), and troponin C (TnC), and each of these components...  Read full blog post.

mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Troponin I Type 3 (cardiac) [p Ser150] Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol TNNI3
Entrez
Uniprot