Human Troponin C (cardiac) ELISA Kit (Colorimetric)

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ELISA: Human Troponin C (cardiac) ELISA Kit (Colorimetric) [NBP2-76596] - Samples were spiked with high concentrations of Human Troponin C (cardiac) and diluted with Reference Standard & Sample Diluent to produce ...read more
ELISA: Human Troponin C (cardiac) ELISA Kit (Colorimetric) [NBP2-76596] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
78.125 - 5000pg/mL
Sensitivity
46.875pg/mL

Order Details

Human Troponin C (cardiac) ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human TNNC1 in samples. No significant cross-reactivity or interference between Human TNNC1 and analogues was observed.
Standard Curve Range
78.125 - 5000pg/mL
Sensitivity
46.875pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.96
Intra-Assay
CV% < 4.82
Spike Recovery
85-102%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TNNC1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Troponin C (cardiac) ELISA Kit (Colorimetric)

  • cardiac troponin C
  • CMD1Z
  • CMH13
  • slow twitch skeletal/cardiac muscle troponin C
  • TNC
  • TN-C
  • TNNC
  • troponin C type 1 (slow)
  • troponin C, slow skeletal and cardiac muscles
  • troponin C, slow
  • troponin C1, slow

Background

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Troponin C (cardiac) ELISA Kit (NBP2-76596). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol TNNC1