TRIM45 Antibody (OTI3G3) - Azide and BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY TRIM45.
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: TRIM45 Antibody (OTI3G3) - Azide and BSA Free [NBP2-74612] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clone
OTI3G3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

TRIM45 Antibody (OTI3G3) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 288-580 of human TRIM45(NP_079464) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TRIM45
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
Theoretical MW
64.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for TRIM45 Antibody (OTI3G3) - Azide and BSA Free

  • RING finger protein 99
  • RNF99FLJ13181
  • tripartite motif containing 45
  • tripartite motif-containing 45
  • tripartite motif-containing protein 45

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRIM45