TRIM45 Antibody (OTI3G3)

Immunohistochemistry: TRIM45 Antibody (OTI3G3) [NBP2-46223] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: TRIM45 Antibody (OTI3G3) [NBP2-46223] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: TRIM45 Antibody (OTI3G3) [NBP2-46223] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: TRIM45 Antibody (OTI3G3) [NBP2-46223] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: TRIM45 Antibody (OTI3G3) [NBP2-46223] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: TRIM45 Antibody (OTI3G3) [NBP2-46223] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunofluorescence: TRIM45 Antibody (OTI3G3) [NBP2-46223] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY TRIM45.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC, ICC/IF
• Clone: OTI3G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TRIM45 Antibody (OTI3G3) Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 288-580 of human TRIM45(NP_079464) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRIM45
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunofluorescence 1:100
  • Immunohistochemistry 1:150
• Theoretical MW: 64.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRIM45 Antibody (OTI3G3)

• RING finger protein 99
• RNF99FLJ13181
• tripartite motif containing 45
• tripartite motif-containing 45
• tripartite motif-containing protein 45

Background

TRIM45 belongs to a family of tripartite motif (TRIM) proteins that play important roles in a variety of cellular functions, including cell proliferation, differentiation, development, oncogenesis, and apoptosis (Wang et al., 2004 [PubMed 15351693]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM45