Home » TRIM35 » TRIM35 Antibodies » TRIM35 Antibody

TRIM35 Antibody

 Submit a Review

Images

 
(
Enlarge)
Western Blot: TRIM35 Antibody [NBP2-88472] - WB Suggested Anti-TRIM35 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate
Western Blot: TRIM35 Antibody [NBP2-88472] - WB Suggested Anti-TRIM35 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate
Immunohistochemistry: TRIM35 Antibody [NBP2-88472] - Immunohistochemistry with Human Small Intestine tissue at an antibody concentration of 5.0ug/ml using anti-TRIM35 antibody

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

TRIM35 Antibody Summary

Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human TRIM35. Peptide sequence: ATKQLCKEFTDLLAQDRTPIGNSRPSPILEPGIQSCLTHFSLITHGFGAP The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM35
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1.0 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TRIM35 Antibody

  • HLS5Hemopoietic lineage switch protein 5
  • KIAA1098MGC17233
  • MAIR
  • tripartite motif containing 35
  • tripartite motif-containing 35
  • tripartite motif-containing protein 35

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

BANP Antibody
NB100-79784
Immunoprecipitation: BANP Antibody [NB100-79784] - Detection of human SMAR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMAR1 antibody NB100-79784 (lot NB100-79784-3) used for IP at 6 ug per reaction. SMAR1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79784-2). For blotting immunoprecipitated SMAR1, NB100-79784 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: BANP Antibody [NB100-79784] - Detection of human and mouse SMAR1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMAR1 antibody NB100-79784 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: BANP Antibody [NB100-79784] - Detection of mouse SMAR1 by immunohistochemistry. Samples: FFPE sections of mouse renal cell carcinoma. Antibodies: Affinity purified rabbit anti-SMAR1 Cat. No. NB100-79784 Lot 2 used at a dilution of 1:5,000 (0.2ug/ml) (left) and Cat. No. NB100-79784 Lot3 used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
DAP12 Antibody
(406288) [Unconjugate
MAB5240
Western blot shows lysates of NS0 mouse myeloma cell line, mock transfected, and primary human CD56+ NK cells. PVDF membrane was probed with 5 µg/mL of Human DAP12 Monoclonal Antibody (Catalog # MAB5240) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DAP12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Human peripheral blood CD56+ natural killer cells were stained with Human DAP12 Monoclonal Antibody (Catalog # MAB5240, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
(2 Publications)
Species: Hu
Applications: CyTOF-ready, ICFlow, WB
GATA-1 Antibody
(4F5)
NBP1-47492
Immunohistochemistry: GATA-1 Antibody (4F5) [NBP1-47492] - Staining of Fetal Baboon Liver. Antigen retrieval: 10mM sodium citrate, pH 6.0, Primary Ab: 1:500 dilution, Secondary Ab (biotinylated): 1:200, Streptavidin-AlexaFluor488: 1:500 (green), Quench autofluorescence with 1% Sudan Black in 70% methanol. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis of K562(left) cells using GATA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis of pancreatic cancer, using GATA1 mouse mAb with DAB staining.
(1 Review)
(2 Publications)
Species: Pm, Hu
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, WB
ZFPM1 Antibody
NBP2-47572
Immunocytochemistry/Immunofluorescence: ZFPM1 Antibody [NBP2-47572] - Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm, cytosol & vesicles.
Immunohistochemistry-Paraffin: ZFPM1 Antibody [NBP2-47572] - Staining of human cervix, uterine shows low expression as expected.
Immunohistochemistry-Paraffin: ZFPM1 Antibody [NBP2-47572] - Staining in human stomach and cervix, uterine tissues using anti-ZFPM1 antibody. Corresponding ZFPM1 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
HEY1 Antibody
NBP2-16818
Western Blot: HEY1 Antibody [NBP2-16818] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Frozen: HEY1 Antibody [NBP2-16818] - Hey1 staining on E11.5 mouse Pharyngeal Mesoderm. Fixed 4% PFA overnight. Blocked with 1% BSA. Primary antibody dilution at 1:100. Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. IHC-Fr image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: HEY1 Antibody [NBP2-16818] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HEY1 protein stained by HEY1 antibody diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Blue: Hoechst 33342 staining.
(1 Review)
Species: Hu
Applications: ICC/IF, IHC, IHC-Fr, WB
SNRPE Antibody
NBP2-43792
Western Blot: SNRPE Antibody [NBP2-43792] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SNRPE antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody
Immunohistochemistry-Paraffin: SNRPE Antibody [NBP2-43792] - Mouse brain. SNRPE stained by SNRPE antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: SNRPE Antibody [NBP2-43792] - Mouse brain.SNRPE stained by SNRPE antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
TRIM17 Antibody
(2E11)
H00051127-M01
Sandwich ELISA: TRIM17 Antibody (2E11) [H00051127-M01] - Detection limit for recombinant GST tagged TRIM17 is 0.03 ng/ml as a capture antibody.
Immunocytochemistry/Immunofluorescence: TRIM17 Antibody (2E11) [H00051127-M01] - Analysis of monoclonal antibody to TRIM17 on HeLa cell . Antibody concentration 10 ug/ml.
(1 Publication)
Species: Hu
Applications: ELISA, ICC/IF, PLA, S-ELISA, WB
ERK1 Antibody (1E5)
NBP2-22203
Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red).
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining.
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
CEBP Beta Antibody
(SC55-05)
NBP2-67635
Flow Cytometry: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Analysis of Hela cells with C/EBP Beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Staining C/EBP Beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Staining C/EBP Beta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IP, WB
SHP-1 Antibody
(SR41-02)
NBP2-67321
Western Blot: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of SHP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: Jurkat cell lysate
Flow Cytometry: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of Hela cells with SHP1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: SHP-1 Antibody (SR41-02) [NBP2-67321] - Staining SHP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
FCER1G Antibody
NBP1-85959
Immunohistochemistry-Paraffin: FCER1G Antibody [NBP1-85959] - Staining of human cerebral cortex shows moderate membranous and cytoplasmic positivity in glial cells.
Immunohistochemistry-Paraffin: FCER1G Antibody [NBP1-85959] - Staining of human kidney shows no positivity in cells in tubules as expected.
Immunohistochemistry-Paraffin: FCER1G Antibody [NBP1-85959] - Staining of human lymph node shows strong membranous positivity in non-germinal center cells.
Species: Hu
Applications: IHC, IHC-P

CD300b/LMIR5/CD300LB A
AF2580
CD300b/LMIR5/CD300LB Antibody
(1 Publication)
Species: Mu
Applications: WB
Erythropoietin/EPO
[HRP]
MEP00B
(3 Reviews)
(94 Publications)
Species: Mu
Applications: ELISA

alpha-Fetoprotein/AFP ...
MAB1368
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and alpha ‑Fetoprotein/AFP knockout HepG2 cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) in the parental HeLacell line, but is not detectable in knockout HeLa cell line. GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
alpha -Fetoprotein/AFP knockout HepG2 hepatocellular carcinoma cell line was stained with Mouse Anti-Human alpha -Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0102B). No staining in the alpha -Fetoprotein/AFP knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
(11 Reviews)
(50 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow, KO, Simple Western, WB

CD300f/LMIR3/CD300LF A
AF2788
CD300f/LMIR3/CD300LF Antibody
(3 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, WB
ERK2 Antibody
(SZ25-01)
NBP2-67360
Flow Cytometry: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of Hela cells with ERK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
(1 Review)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB

Publications for TRIM35 Antibody (NBP2-88472) (0)

There are no publications for TRIM35 Antibody (NBP2-88472).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TRIM35 Antibody (NBP2-88472) (0)

There are no reviews for TRIM35 Antibody (NBP2-88472). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TRIM35 Antibody (NBP2-88472) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional TRIM35 Products

Bioinformatics Tool for TRIM35 Antibody (NBP2-88472)

Discover related pathways, diseases and genes to TRIM35 Antibody (NBP2-88472). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TRIM35 Antibody (NBP2-88472)

Discover more about diseases related to TRIM35 Antibody (NBP2-88472).
 

Pathways for TRIM35 Antibody (NBP2-88472)

View related products by pathway.

Research Areas for TRIM35 Antibody (NBP2-88472)

Find related products by research area.

Blogs on TRIM35

There are no specific blogs for TRIM35, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TRIM35 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol TRIM35