TREX2 Antibody

Immunohistochemistry-Paraffin: TREX2 Antibody [NBP1-36998] - 3.8ug/ml Staining of Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TREX2 Antibody Summary

• Immunogen: Peptide with sequence C-HRSSLENPEHDES corresponding to internal region according to NP_542432.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TREX2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1:100 - 1:2000
• Application Notes: WB: Preliminary experiments gave an approx. 18 kDa band in lysates of Jurkat, MOLT4 and U937 after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25.9 kDa band according to NP_542432.2. The 18 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human breast shows nuclear staining in the luminal epithelial duct cells.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TREX2 Antibody

• 3'-5' exonuclease TREX2
• EC 3.1.11.2
• three prime repair exonuclease 2,3'-5' exonuclease TREX2 long form

Background

Please note, this product is one of a range of Aspiring Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot. However no additional claims are made for their ability to recognise native protein in any application.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TREX2 Antibody (NBP1-36998)(1)

Publication using NBP1-36998

• de Silva U, Choudhury S, Bailey SL et al. The crystal structure of TREX1 explains the 3' nucleotide specificity and reveals a polyproline II helix for protein partnering. J Biol Chem 2007-04-01 [PMID: 17293595]

Bioinformatics

• Gene Symbol: TREX2
• Entrez:
  • 11219(Human)
• Uniprot:
  • NP_542432.2(Human)