TRBP Antibody

Western Blot: TRBP Antibody [NB100-56471] - Analysis of TRBP2 in the 1) absence and 2) presence of immunizing peptide in human brain lysate using this antibody at 0.5 ug/ml.

Product Discontinued

TRBP Antibody Summary

• Immunogen: A portion of amino acids 150-200 of human TRBP2 was used as the immunogen.
• Specificity: Human isoform a is a 366 amino acid protein, and human isoform b is a 345 amino acid protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TARBP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRBP Antibody

• LOQS
• RISC-loading complex subunit TARBP2
• TAR (HIV) RNA binding protein 2
• TAR (HIV) RNA-binding protein 2
• TAR (HIV) RNA-binding protein TRBP1
• TAR (HIV-1) RNA binding protein 2
• TAR RNA binding protein 2
• TAR RNA-binding protein 2
• trans-activation responsive RNA-binding protein
• Trans-activation-responsive RNA-binding protein
• TRBP
• TRBP1
• TRBP2

Background

TRBP belongs to the family of dsRNA-binding proteins. It consists of highly conserved 2 dsRNA-binding domains and C-terminal basic region. It is a unique cellular protein capable of binding to HIV-TAR RNA and stimulates expression of HIV-1 long terminal repeats. It also inhibits the host antiviral and anti-proliferative mechanisms by directly binding to IFN-regulated dsRNA-dependent protein kinase (PKR). TRBP associates with Ago2 protein and forms an integral component of Dicer-containing complex. It has a role in micro RNA processing and in RISC assembly. Other functions of TRBP include promoting cellular growth and tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRBP Antibody (NB100-56471)(1)

Publication using NB100-56471

• Zhang H, Laux A, Stenmark K, Hu C Mechanisms Contributing to the Dysregulation of miRNA-124 in Pulmonary Hypertension International Journal of Molecular Sciences 2021-04-08 [PMID: 33917769] (WB, Human)

Bioinformatics

• Gene Symbol: TARBP2
• Entrez:
  • 6895(Human)
  • 21357(Mouse)
  • 363006(Rat)
• Uniprot:
  • Q15633(Human)