Recombinant Human TPK1 His Protein

SDS-Page: Recombinant Human TPK1 Protein [NBP1-99102] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human TPK1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-243 of Human TPK1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMEHAFT PLEPLLSTGN LKYCLVILNQ PLDNYFRHLW NKALLRACAD GGANRLYDIT EGERESFLPE FINGDFDSIR PEVREYYATK GCELISTPDQ DHTDFTKCLK MLQKKIEEKD LKVDVIVTLG GLAGRFDQIM ASVNTLFQAT HITPFPIIII QEESLIYLLQ PGKHRLHVDT GMEGDWCGLI PVGQPCMQVT TTGLKWNLTN DVLAFGTLVS TSNTYDGSGV VTVETDHPLL WTMAIKS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TPK1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 29.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human TPK1 His Protein

• EC 2.7.6.2
• HTPK1
• Placental protein 20
• PP20thiamine kinase
• thiamin pyrophosphokinase 1
• thiamine diphosphokinase
• Thiamine pyrophosphokinase 1

Background

TPK1, also known as thiamin pyrophosphokinase 1, catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. This protein can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. Recombinant human TPK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for TPK1 Recombinant Protein (NBP1-99102)(1)

Publication using NBP1-99102

• Vora B Integration of benchwork, clinical trials, and real world data to investigate drug interactions Thesis 2021-01-01

FAQs for TPK1 Recombinant Protein (NBP1-99102). (Showing 1 - 1 of 1 FAQ).

1. We are trying to get activity out of this enzyme (thiamine pyrophosphokinase) and are having difficulty. Do you guys have any recommendations that may help us out?
   • I am sorry for any confusion regarding our protein product NBP1-99102. This antibody is sold with a reducing agent present in the buffer as the protein is advertised for use in SDS PAGE gels as a positive control. Our lab has not yet tested this protein for use in functional assays that require activity. 

Bioinformatics

• Gene Symbol: TPK1