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TOX2 Antibody

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Western Blot: TOX2 Antibody [NBP2-94397] - Analysis of extracts of various cell lines, using TOX2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.
Western Blot: TOX2 Antibody [NBP2-94397] - Analysis of extracts of various cell lines, using TOX2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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TOX2 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human TOX2 (NP_116272.1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TOX2
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for TOX2 Antibody

  • C20orf100
  • chromosome 20 open reading frame 100
  • dJ495O3.1
  • GCX1
  • GCX-1dJ1108D11.2
  • granulosa cell HMG box 1
  • Granulosa cell HMG box protein 1
  • granulosa cell HMG-box protein 1
  • MGC15880
  • TOX high mobility group box family member 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ST5 Antibody
NBP2-85831
Immunohistochemistry-Paraffin: ST5 Antibody [NBP2-85831] - Rabbit Anti-ST5 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Heart Tissue. Observed Staining: Cytoplasm in cardiomyocytes. Primary Antibody Concentration: N/A. Other Working Concentrations: 1:600. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20X. Exposure Time: 0.5 - 2.0 sec
Western Blot: ST5 Antibody [NBP2-85831] - WB Suggested Anti-ST5 Antibody. Titration: 1.0 ug/ml. Positive Control: 721_B Whole Cell
Immunohistochemistry-Paraffin: ST5 Antibody [NBP2-85831] - Rabbit Anti-ST5 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue. Observed Staining: Cytoplasmic, nucleus. Primary Antibody Concentration: N/A. Other Working Concentrations: 1:600. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20X. Exposure Time: 0.5 - 2.0 sec
Species: Hu
Applications: IHC, IHC-P, WB
TOX3 Antibody
(TOX3/1123)
NBP2-45165
Immunofluorescence: TOX3 Antibody (TOX3/1123) [NBP2-45165] - E4.5 embryonic chicken neural tube. TOX3 antibody at 1:200 dilution. IF image submitted by a verified customer review.
Western Blot: TOX3 Antibody (TOX3/1123) [NBP2-45165] - Western Blot Analysis of A431 cell lysate using TOX3 Mouse Monoclonal Antibody (TOX3/1123).
Protein Array: TOX3 Antibody (TOX3/1123) [NBP2-45165] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TOX3 Mouse Monoclonal Antibody (TOX3/1123).
(1 Review)
Species: Ch, Hu
Applications: ELISA, IF, IHC, IHC-P, PA, WB
UMODL1 Antibody
NBP1-80535
Western Blot: UMODL1 Antibody [NBP1-80535] - HT1080 cell lysate, concentration 0.2-1 ug/ml.
Species: Hu
Applications: WB
TRIM40 Antibody
H00135644-B01P
Immunocytochemistry/Immunofluorescence: TRIM40 Antibody [H00135644-B01P] - Analysis of purified antibody to TRIM40 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: TRIM40 Antibody [H00135644-B01P] - Analysis of TRIM40 expression in transfected 293T cell line by TRIM40 polyclonal antibody. Lane 1: TRIM40 transfected lysate(28.38 KDa). Lane 2: Non-transfected lysate.
Western Blot: TRIM40 Antibody [H00135644-B01P] - Analysis of TRIM40 expression in K-562.
Species: Hu
Applications: ICC/IF, WB
APCDD1 Antibody -
BSA Free
NB110-92756
Western Blot: APCDD1 Antibody [NB110-92756] - Detection of APCDD1 in human heart lysate
(1 Publication)
Species: Ch, Hu, Mu, Re
Applications: WB
Fatty Acid
Synthase/FASN Antibod
NB400-114
Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle. Confocal section of CHIKV replicon-infected HeLa cells labelled for FASN, dsRNA and 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/ncomms11320), licensed under a CC-BY licence.
Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Analysis of Fatty Acid Synthase, using NB400-114. Samples: 50 ug of total mouse liver lysate.
Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NB400-114] - Immunohistochemical localization of FAS in chicken hypothalmus. Paraffin sections were obtained from 3-wk-old broiler chickens.
(2 Reviews)
(23 Publications)
Species: Ch, Fe, Ha, Hu, Mu, Pl, Po, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, IP, KD, WB
TOX Antibody
NBP1-87857
Western Blot: TOX Antibody [NBP1-87857] - Analysis in human cell line MOLT-4.
Immunohistochemistry-Paraffin: TOX Antibody [NBP1-87857] - Staining of human pancreas shows nuclear positivity in islets of Langerhans.
Immunohistochemistry-Paraffin: TOX Antibody [NBP1-87857] - Staining in human tonsil and liver tissues. Corresponding TOX RNA-seq data are presented for the same tissues.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
SSRP1 Antibody
NBP2-80439
Flow Cytometry: SSRP1 Antibody [NBP2-80439] - Analysis of SSRP1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).
Immunohistochemistry-Paraffin: SSRP1 Antibody [NBP2-80439] - Analysis in rat testis tissue using SSRP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with dd
Immunohistochemistry-Paraffin: SSRP1 Antibody [NBP2-80439] - Analysis in human skin tissue using SSRP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with dd
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
CD28 Antibody
(37407) [Unconjugated
MAB342
Human CD28 Monoclonal Antibody enhances IL-2 secretion in the Jurkat human acute T cell leukemia cell line stimulated with 10 ng/mL phorbol myristate acetate (PMA) and 0.5 μM calcium ionophore, in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (Catalog # D2050). The ED50 for this effect is typically 0.2-0.6 μg/mL.
(2 Reviews)
(33 Publications)
Species: Hu
Applications: AgAct, WB
Galectin-4
Antibody (9Z2B5)
NBP3-16249
Immunohistochemistry-Paraffin: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunohistochemistry of paraffin-embedded human appendix using Galectin-4 Rabbit mAb (NBP3-16249) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunohistochemistry of paraffin-embedded mouse colon using Galectin-4 Rabbit mAb (NBP3-16249) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunofluorescence analysis of rat rectum using Galectin-4 Rabbit mAb (NBP3-16249) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
SOHLH1 Antibody
NBP1-56454
Western Blot: SOHLH1 Antibody [NBP1-56454] - Hela cell lysate, concentration 0.2-1 ug/ml.
Species: Hu
Applications: WB
MID1 Antibody
NBP3-05061
Immunocytochemistry/Immunofluorescence: MID1 Antibody [NBP3-05061] - Analysis of MCF-7 cells using MID1 antibody . Blue: DAPI for nuclear staining.
Western Blot: MID1 Antibody [NBP3-05061] - analysis of extracts of HeLa cells, using MID1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.
Species: Hu, Mu
Applications: ICC/IF, WB
c-Rel Antibody
[Unconjugated]
AF2699
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and CH‑1 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse c-Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for c-Rel at approximately 69 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Jurkat human acute T cell leukemia cell line treated with 50 ng/mL and 200 ng/mL PMA and calcium ionomycin for overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. c‑Rel/DNA complexes were immuno­precipitated using 5 μg Goat Anti-Human/Mouse c‑Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR.
Simple Western lane view shows lysates of CH‑1 mouse B cell lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Rel at approximately 71 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse c‑Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
(2 Reviews)
(2 Publications)
Species: Hu, Mu
Applications: ChIP, ICC, Simple Western, WB
GDF-9
[Unconjugated]
739-G9/CF
Recombinant Mouse GDF-9 induces Mv1Lu mink lung epithelial cell death. The ED50 for this effect is 0.1-0.4 μg/mL.
(12 Publications)
Species: Mu
Applications: BA
Mouse CD3+ T Cell,
Small Column Enrichme ...
MTCC-10-NOV
Mouse CD3+ T Cell, Small Column Enrichment Kit
Species: Mu
ARF1 Antibody (1D9)
NB300-505
Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of 3T3 cells compared to an isotype control (blue).
Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of Hela cells compared to an isotype control (blue).
Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of MCF-7 cells compared to an isotype control (blue).
(13 Publications)
Species: Bv, Ca, Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, WB
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.
Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
(9 Reviews)
(37 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
TRIM61 Antibody
NBP2-13485
Immunohistochemistry-Paraffin: TRIM61 Antibody [NBP2-13485] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunocytochemistry/Immunofluorescence: TRIM61 Antibody [NBP2-13485] - Staining of human cell line U-2 OS shows localization to nucleoli fibrillar center & endoplasmic reticulum.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
TOX4 Antibody
NBP1-88433
Immunocytochemistry/Immunofluorescence: TOX4 Antibody [NBP1-88433] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Western Blot: TOX4 Antibody [NBP1-88433] - Analyisis in in mouse cell line NIH-3T3 and rat cell line NBT-II
Western Blot: TOX4 Antibody [NBP1-88433] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MIS/AMH
[Unconjugated]
1737-MS/CF
MIS/AMH [Unconjugated]
(14 Publications)
Species: Hu
Applications: Bind

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Bioinformatics

Gene Symbol TOX2