This F11 antibody has been tested by western blot analysis of reduced cell lysates prepared from Jurkat cells. This can be used at 1-5 ug/mL. This F11 antibody has also been reported for use in immunoprecipitation and immunohistology staining of paraffin embedded tissue sections. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Read Publication using NBP2-00217 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
PBS (pH 7.2)
0.09% Sodium Azide
Protein A or G purified
Alternate Names for TOR/mTOR Antibody (F11)
FK506 binding protein 12-rapamycin associated protein 1
FK506 binding protein 12-rapamycin associated protein 2
FK506-binding protein 12-rapamycin complex-associated protein 1
FKBP12-rapamycin complex-associated protein
FRAP1FKBP12-rapamycin complex-associated protein 1
Mammalian target of rapamycin
mechanistic target of rapamycin (serine/threonine kinase)
Mechanistic target of rapamycin
rapamycin and FKBP12 target 1
rapamycin associated protein FRAP2
Rapamycin target protein 1
RAPT1FKBP-rapamycin associated protein
serine/threonine-protein kinase mTOR
This F11 monoclonal antibody reacts with human, mouse, and rat mammalian target of rapamycin (mTOR). This 289-kDa serine/threonine kinase is activated via phosphorylation at Ser2448 by the PI3K/Akt signaling pathway in response to growth factors, energy, nutrients, and stress. mTOR is highly conserved amongst eukaryotes and constitutively expressed in immune (e.g. T, B, NK, dendritic cells, mast cells, and neutrophils) and non-immune tissues (e.g. heart). mTOR exists as a member of two distinct complexes, mTORC1 and mTORC2, which have differing sensitivities to rapamycin and phosphorylation targets. Rapamycin inhibits mTOR activity by binding FKBP12, which causes dissociation of mTORC1 and mTORC2. This molecule plays a critical role in cell metabolism, survival, dendritic cell maturation and activation, and protein synthesis. Deregulation of mTOR has been implicated in a variety of cancers. Studies have also suggested a role for mTOR in Foxp3 expression and regulatory T cell differentiation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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