TOMM22 Antibody (4G4)

Western Blot: TOMM22 Antibody (4G4) [H00056993-M01] - TOMM22 monoclonal antibody (M01), clone 4G4 Analysis of TOMM22 expression in NIH/3T3.

Immunocytochemistry/Immunofluorescence: TOMM22 Antibody (4G4) [H00056993-M01] - Analysis of monoclonal antibody to TOMM22 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: TOMM22 Antibody (4G4) [H00056993-M01] - Analysis of monoclonal antibody to TOMM22 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.

Western Blot: TOMM22 Antibody (4G4) [H00056993-M01] - Analysis of TOMM22 over-expressed 293 cell line, cotransfected with TOMM22 Validated Chimera RNAi ( Cat # H00056993-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TOMM22 monoclonal antibody (M01), clone 4G4 (Cat # H00056993-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: TOMM22 Antibody (4G4) [H00056993-M01] - TOMM22 monoclonal antibody (M01), clone 4G4. Analysis of TOMM22 expression in PC-12.

Western Blot: TOMM22 Antibody (4G4) [H00056993-M01] - TOMM22 monoclonal antibody (M01), clone 4G4. Analysis of TOMM22 expression in Raw 264.7.

Western Blot: TOMM22 Antibody (4G4) [H00056993-M01] - Analysis of TOMM22 expression in transfected 293T cell line by TOMM22 monoclonal antibody (M01), clone 4G4.Lane 1: TOMM22 transfected lysate(15.5 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: TOMM22 Antibody (4G4) [H00056993-M01] - Detection limit for recombinant GST tagged TOMM22 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, RNAi, S-ELISA
• Clone: 4G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TOMM22 Antibody (4G4) Summary

• Immunogen: TOMM22 (AAH09363, 1 a.a. ~ 142 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAAVAAAGAGEPQSPDELLPKGDAEKPEEELEEDDDEELDETLSERLWGLTEMFPERVRSAAGATFDLSLFVAQKMYRFSRAALWIGTTSFMILVLPVVFETEKLQMEQQQQLQQRQILLGPNTGLSGGMPGALPSLPGKI
• Specificity: TOMM22 - translocase of outer mitochondrial membrane 22 homolog (yeast)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TOMM22
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • RNA Inhibition
  • Sandwich ELISA
• Application Notes: Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation and ELISA.

Reactivity Notes

Human, mouse & rat. Other species have not been tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TOMM22 Antibody (4G4)

• 1C9-2
• hTom22
• mitochondrial import receptor subunit TOM22 homolog
• mitochondrial import receptor Tom22
• MST065
• TOM22MSTP065
• Translocase of outer membrane 22 kDa subunit homolog
• translocase of outer mitochondrial membrane 22 homolog (yeast)

Background

The protein encoded by this gene is an integral membrane protein of the mitochondrial outer membrane. The encoded protein interacts with TOMM20 and TOMM40, and forms a complex with several other proteins to import cytosolic preproteins into the mitochondrion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TOMM22 Antibody (H00056993-M01)(1)

Publication using H00056993-M01

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018

Bioinformatics

• Gene Symbol: TOMM22
• Entrez:
  • 56993(Human)
• OMIM:
  • 607046(Human)
• Uniprot:
  • AAH09363(Human)