TOMM20 Antibody (1074702) [Unconjugated]

TOMM20 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human TOMM20 Monoclonal Antibody (Catalog # MAB11604) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the mitochondrion membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human TOMM20 Monoclonal Antibody (Catalog # MAB11604) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TOMM20 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

TOMM20 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human TOMM20 Monoclonal Antibody (Catalog # MAB11604) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of bronchial epithelium. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

TOMM20 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human TOMM20 Monoclonal Antibody (Catalog # MAB11604) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial and Leydig cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 1074702
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TOMM20 Antibody (1074702) [Unconjugated] Summary

• Immunogen: TOMM20 containing synthetic peptide
  Accession # Q15388
• Specificity: Detects a synthetic peptide specific for human TOMM20 containing amino acid 55 in Direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TOMM20 Antibody (1074702) [Unconjugated]

• KIAA0016Outer mitochondrial membrane receptor Tom20
• MAS20
• MGC117367
• mitochondrial 20 kDa outer membrane protein
• mitochondrial import receptor subunit TOM20 homolo
• mitochondrial import receptor subunit TOM20 homolog
• MOM19
• outer mitochondrial membrane receptor Tom20
• TOM20
• TOMM20
• translocase of outer mitochondrial membrane 20 hom
• translocase of outer mitochondrial membrane 20 homolog (yeast)
• translocase of outer mitochondrial membrane 20 homolog type II

Background

TOMM20 (Translocase of Outer Mitochondrial Membrane 20) is a receptor and key unit of the TOM complex.  The TOM complex translocates proteins produced from nuclear DNA through the mitochondrial membrane for use in oxidative phosphorylation.  TOMM20 is a conserved outer membrane protein and depletion of TOMM20 produces a large number of dysfunctional mitochondria with the loss of membrane potential.  Increased TOMM20 expression has been associated with malignancy of various cancers and contributes to tumorigenesis.

1. Park SH, Lee AR, Choi K, Joung S, Yoon JB, Kim S. TOMM20 as a potential therapeutic target of colorectal cancer. BMB Rep. 2019 Dec;52(12):712-717. doi: 10.5483/BMBRep.2019.52.12.249. PMID: 31818360; PMCID: PMC6941759.Nijenjuis M., Hämmerling GJ.  J Immunol. 1996 Dec 15;157(12):5467-77.
2. Yin L, Ye Y, Zou L, Lin J, Dai Y, Fu Y, Liu Y, Peng Y, Gao Y, Fu Y, Qi X, Deng T, Zhang S, Li X. AR antagonists develop drug resistance through TOMM20 autophagic degradation-promoted transformation to neuroendocrine prostate cancer. J Exp Clin Cancer Res. 2023 Aug 10;42(1):204. doi: 10.1186/s13046-023-02776-0. PMID: 37563661; PMCID: PMC10413764.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q15388(Human)