TIP120B Antibody

Product Discontinued

TIP120B Antibody Summary

• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1130-1143 of Human CAND2/TIP120B (C-terminal) coupled to KLH.
• Specificity: This product reacts with human, rat and mouse CAND2/TIP120B. Cross reactivity does occur with human, rat and mouse CAND1/TIP120A.
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAND2
• Purity: Sterile filtered

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • Western Blot 1:500-1:1000
• Application Notes: The antibody immunoprecipitates in vitro translated protein and protein from transfected cell lysates . Coimmunoprecipitation of related proteins has not been tested. A 125.4 kDa band corresponding - human CAND2 is detected. CAND2 is specifically expressed in muscle and heart tissue. Researchers should determine optimal titers for other applications.

Reactivity Notes

Cross reactivity with TIP120B from other sources has not been tested.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Antiserum
• Preservative: 0.01% Sodium Azide
• Purity: Sterile filtered

Alternate Names for TIP120B Antibody

• cullin-associated and neddylation-dissociated 2 (putative)
• Cullin-associated and neddylation-dissociated protein 2
• cullin-associated NEDD8-dissociated protein 2
• epididymis tissue protein Li 169
• KIAA0667TBP-interacting protein of 120 kDa B
• p120 CAND2
• TBP interacting protein
• TBP-interacting protein 120B
• TIP120BTp120b

Background

CAND2 is also known as TIP120B, and TATA-binding protein-interacting protein 120B. While both CAND1 (TIP120A) and CAND2 (TIP120B) are TATA-binding proteins and form complexes with various nuclear proteins involved in the control of eukaryotic gene transcription, CAND2 (TIP120B) is expressed specifically in muscle and heart tissue. This is contrary to the ubiquitous expression of CAND1 (TIP120A). TIP120 homologs exist in various higher eukaryotes including D. melanogaster, C. elegans, and A. thaliana. TIP120B is 60% identical in amino acid sequence to TIP120A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIP120B Antibody (NBP1-78048)(3)

Publications using NBP1-78048

• Feng S, Shen Y, Sullivan JA et al. Arabidopsis CAND1, an unmodified CUL1-interacting protein, is involved in multiple developmental pathways controlled by ubiquitin/proteasome-mediated protein Degradation. Plant Cell 2004-07-01 [PMID: 15208391]
• Cheng Y, Dai X, Zhao Y et al. AtCAND1, a HEAT-repeat protein that participates in auxin signaling in Arabidopsis. Plant Physiol 2004-06-01 [PMID: 15181201]
• Liu J, Furukawa M, Matsumoto T et al. NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Mol Cell 2002-12-01 [PMID: 12504025]

Bioinformatics

• Gene Symbol: CAND2
• Uniprot:
  • O75155(Human)