Thymopoietin/LAP2 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Thymopoietin/LAP2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Thymopoietin
  Pro2-Gln187
• Specificity: Detects human Thymopoietin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TMPO
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thymopoietin/LAP2 Antibody [Unconjugated]

• CMD1T
• lamina-associated polypeptide 2
• LAP2PRO0868
• LEM domain containing 4
• LEMD4
• MGC61508
• Thymopoietin isoform alpha
• Thymopoietin
• Thymopoietin, isoforms beta/gamma
• Thymopoietin-related peptide isoform alpha
• Thymopoietin-related peptide isoforms beta/gamma
• TMPO
• TP alpha
• TP beta/gamma
• TP
• TPRP isoform alpha
• TPRP isoforms beta/gamma

Background

Thymopoietin (TP alpha; also TMPO, LAP2 alpha and TPRP alpha ) is a 75 kDa member of the LEM family of molecules.  It is a nucleoplasmic protein that is widely expressed in cells such as keratinocytes and thymocytes.  Here, it interacts with A-type lamins and Rb/retinoblastoma protein, thus participating in cell cycle exit.  Human thymopoietin is 694 amino acids (aa) in length.  It contains a potential N-terminal “thymopoietin peptide” segment (aa #2-49), a BAF-binding LEM domain (aa #109-153), an NLS (aa #190-196), and a coiled-coil region (aa #558-657).  Multiple splice variants exist.  All contain the same 188 N-terminal amino acids.  Five variants show singular 49, 157, 266, 226, and 60 aa substitutions for the C-terminal 506 amino acids.  Over aa #2-187, human thymopoietin shares 93% aa identity with mouse thymopoietin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Thymopoietin/LAP2 Antibody (AF843)(2)

Publications using AF843

• Cheng, S;Saliba, N;Gagliano, G;Joshi, P;Gustavsson, AK; Single-objective lattice light sheet microscopy with microfluidics for single-molecule super-resolution imaging of mammalian cells bioRxiv : the preprint server for biology 2025-07-23 [PMID: 40777334] (Immunocytochemistry, Human)
• Saliba, N;Gagliano, G;Gustavsson, AK; Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet bioRxiv : the preprint server for biology 2023-09-29 [PMID: 37808751] (ICC, Human)

Bioinformatics

• Gene Symbol: TMPO