Thymopoietin/LAP2 Antibody [Alexa Fluor® 750]

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 750

Thymopoietin/LAP2 Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human Thymopoietin
  Pro2-Gln187
• Specificity: Detects human Thymopoietin in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Thymopoietin (TP alpha; also TMPO, LAP2 alpha and TPRP alpha ) is a 75 kDa member of the LEM family of molecules.  It is a nucleoplasmic protein that is widely expressed in cells such as keratinocytes and thymocytes.  Here, it interacts with A-type lamins and Rb/retinoblastoma protein, thus participating in cell cycle exit.  Human thymopoietin is 694 amino acids (aa) in length.  It contains a potential N-terminal “thymopoietin peptide” segment (aa #2-49), a BAF-binding LEM domain (aa #109-153), an NLS (aa #190-196), and a coiled-coil region (aa #558-657).  Multiple splice variants exist.  All contain the same 188 N-terminal amino acids.  Five variants show singular 49, 157, 266, 226, and 60 aa substitutions for the C-terminal 506 amino acids.  Over aa #2-187, human thymopoietin shares 93% aa identity with mouse thymopoietin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.