Thioredoxin-2 Antibody [Alexa Fluor® 405]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

Thioredoxin-2 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant mouse Thioredoxin-2
  Thr60-Gly166
  Accession # P97493
• Specificity: Detects endogenous human, mouse and rat Thioredoxin 2 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thioredoxin-2 Antibody [Alexa Fluor® 405]

• mitochondrial thioredoxin
• MTRX
• MT-TRX
• thioredoxin 2
• thioredoxin, mitochondrial
• Thioredoxin2
• Thioredoxin-2
• Trx2
• TXN2

Background

Thioredoxin-2 (Trx2), also known as mitochondrial Thioredoxin, belongs to the evolutionarily conserved Thioredoxin family. These proteins share the Thioredoxin fold containing the active site-CGPC motif. In their reduced form, the active site cysteine residues reduce protein disulfides. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by a NADPH-dependent Thioredoxin reductase. Thioredoxin-2 contains an N-terminal 59 amino acid (aa) transit sequence that is cleaved upon translocation to mitochondria. The amino acid sequence of mature mouse Thioredoxin‑2 is identical to mature rat Thioredoxin-2 and 98% identical to human Thioredoxin-2. Thioredoxin-2 interacts with specific components of the mitochondrial respiratory chain and helps regulate the membrane potential. Thioredoxin-2 is ubiquitously but variably expressed and high expression confers resistance to oxidant‑induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.