Thioredoxin-2 Antibody (543317) [Alexa Fluor® 647]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 543317
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Thioredoxin-2 Antibody (543317) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human Thioredoxin-2
  Thr60-Gly166
  Accession # Q99757
• Specificity: Detects human, mouse and rat Thioredoxin-2 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thioredoxin-2 Antibody (543317) [Alexa Fluor® 647]

• mitochondrial thioredoxin
• MTRX
• MT-TRX
• thioredoxin 2
• thioredoxin, mitochondrial
• Thioredoxin2
• Thioredoxin-2
• Trx2
• TXN2

Background

Thioredoxin-2 (Trx-2; also known as mitochondrial Thioredoxin) belongs to the evolutionarily conserved Thioredoxin family of proteins. Thioredoxins share the Thioredoxin fold containing the active site-CGPC motif. In their reduced form, the active site cysteine residues reduce protein disulfides. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by a NADPH-dependent Thioredoxin reductase. Thioredoxin-2 contains an N-terminal 59 amino acid (aa) transit sequence that is cleaved upon translocation to mitochondria. The amino acid sequence of mature human Thioredoxin-2 is 98% identical to mouse and rat Thioredoxin-2. Thioredoxin-2 interacts with specific components of the mitochondrial respiratory chain and helps regulate the membrane potential. Thioredoxin-2 is ubiquitously but variably expressed and high expression confers resistance to oxidant-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.