Thioredoxin-1 Antibody (2A7) - Azide and BSA Free

Western Blot: Thioredoxin Antibody (2A7) [H00007295-M01] - TXN monoclonal antibody (M01), clone 2A7 Analysis of TXN expression in HeLa.

Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody (2A7) [H00007295-M01] - Analysis of monoclonal antibody to TXN on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: Thioredoxin Antibody (2A7) [H00007295-M01] - Analysis of TXN expression in transfected 293T cell line by TXN monoclonal antibody (M01), clone 2A7.Lane 1: TXN transfected lysate(11.7 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: Thioredoxin Antibody (2A7) [H00007295-M01] - Analysis of TXN transfected lysate using anti-TXN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TXN MaxPab rabbit polyclonal antibody.

ELISA: Thioredoxin Antibody (2A7) [H00007295-M01] - Detection limit for recombinant GST tagged TXN is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 2A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Thioredoxin-1 Antibody (2A7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TXN (AAH03377, 1 a.a. ~ 105 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVKQIESKTAFQEALDAAGDKLVVVDFSATWCGPCKMIKPFFHSLSEKYSNVIFLEVDVDDCQDVASECEVKCMPTFQFFKKGQKVGEFSGANKEKLEATINELV
• Localization: Cytoplasmic and Secreted.
• Specificity: TXN - thioredoxin
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TXN
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Thioredoxin-1 Antibody (2A7) - Azide and BSA Free

• Thioredoxin1
• Thioredoxin-1
• TRDX;TRX;TRX1;Trx80;TXN
• Trx1
• TXN
• TXN1

Background

Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It is ubiquitous and found in many organisms from plants and bacteria to mammals. Multiple in vitro substrates for thioredoxin have been identified, including ribonuclease, choriogonadotropins, coagulation factors, glucocorticoid receptor, and insulin. Reduction of insulin is classically used as an activity test.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Thioredoxin Antibody (H00007295-M01)(3)

Publications using H00007295-M01

• Romanello KS, Teixeira KKL, Silva JPMO et al. Global analysis of erythroid cells redox status reveals the involvement of Prdx1 and Prdx2 in the severity of beta thalassemia. PLoS One 2018-12-06 [PMID: 30521599]
• Ciamporcero E, Daga M, Pizzimenti S et al. Crosstalk between Nrf2 and YAP contributes to maintaining the antioxidant potential and chemoresistance in bladder cancer. Free Radic. Biol. Med. 2017-12-14 [PMID: 29248722] (Human)
• Ahrens TD, Timme S, Ostendorp J et al. Response of esophageal cancer cells to epigenetic inhibitors is mediated via altered thioredoxin activity. Lab Invest 2016-03-01 [PMID: 26692290]

Bioinformatics

• Gene Symbol: TXN
• Entrez:
  • 7295(Human)
• OMIM:
  • 187700(Human)
• Uniprot:
  • AAH03377(Human)