TGM6 Antibody

Western Blot: TGM6 Antibody [NBP2-75688] - Analysis of TGM6 on different tissue lysates using anti-TGM6 antibody at 1/100 dilution.Positive control:Lane 1 : Mouse brain Lane 2 : Mouse brainLane 3 : Mouse spleen Lane 4 : Mouse spleenLane 5 : Mouse testis

Immunocytochemistry/Immunofluorescence: TGM6 Antibody [NBP2-75688] - Staining TGM6 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: TGM6 Antibody [NBP2-75688] - Analysis of paraffin-embedded rat brain tissue using anti-TGM6 antibody. Counter stained with hematoxylin.

Flow Cytometry: TGM6 Antibody [NBP2-75688] - Analysis of 293T cells with TGM6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: TGM6 Antibody [NBP2-75688] - Staining TGM6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: TGM6 Antibody [NBP2-75688] - Staining TGM6 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: TGM6 Antibody [NBP2-75688] - Analysis of paraffin-embedded human kidney tissue using anti-TGM6 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TGM6 Antibody [NBP2-75688] - Analysis of paraffin-embedded mouse testis tissue using anti-TGM6 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TGM6 Antibody [NBP2-75688] - Analysis of paraffin-embedded mouse brain tissue using anti-TGM6 antibody. Counter stained with hematoxylin.

Product Discontinued

TGM6 Antibody Summary

• Immunogen: Synthetic peptide corresponding to N-terminal of Mouse TGM6 (SwissProt: O95932 Human; SwissProt: Q6Y684 Mouse; SwissProt: F1M4T7 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TGM6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TGM6 Antibody

• SCA35
• TGase-6
• TGM3L
• TGM6
• TGY
• Transglutaminase 6

Background

TGM6 catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of thepyruvate dehydrogenase complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TGM6