TfR (Transferrin R) Antibody (YTA74.4) - BSA Free

Flow Cytometry: TfR (Transferrin R) Antibody (YTA74.4) [NB100-65397] - Alpha4 and beta1 integrin activity regulation is required for erythroblast developmental transition. (A) Representative FACS plots of bone marrow (BM) Ter119+ erythroblasts after treatment with anti-alpha4 blocking antibodies (Abs) or with isotype-matched Abs. Percentages shown are of parent populations. (B) and (C) Histograms showing the mean of two independent experiments for mice treated with anti-alpha4, anti-beta1, their combination or with iso-type-matched Abs.

Flow Cytometry: TfR (Transferrin R) Antibody (YTA74.4) [NB100-65397] - SWAP-70-deficient mice have lower erythropoietic reserves. Upper plots: Ter119(low)CD71(high) comprise proerythroblasts and Ter119+ comprise total erythroblasts. Percentages shown are of total BM and spleen cells. Lower plots: erythroblasts, subgated Ter119high. Percentages shown are of parent populations. (C) Automated-quantified (MACSQuant, Miltenyi) total numbers of Ter119+ cells in the BM (per 1 femur and 1 tibia) and in the spleen of wt and Swap-70(-/-) mice of different ages.

Product Discontinued

TfR (Transferrin R) Antibody (YTA74.4) - BSA Free Summary

• Immunogen: Mouse Concanavilin A activated spleen cells.
• Localization: Type II membrane protein. Secreted (isoform sTfR).
• Marker: Recycling Endosome Marker
• Specificity: Mouse CD71 antigen, the transferrin receptor (TFR). Expressed on all dividing cells and can distinguish resting and activated T-cells. Immunoprecipitates a disulphide linked homodimer of 90kD by SDS PAGE. The antibody inhibits cell proliferation and the mixed lymphocyte response in vitro. Blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies (1,2).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TFRC
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TfR (Transferrin R) Antibody (YTA74.4) - BSA Free

• CD71 antigen
• CD71
• CD71TRFR
• p90
• T9
• TfR (Transferrin R)
• TfR (TransferrinR)
• TfR
• TFR1
• TFRC
• TR
• transferrin receptor (p90, CD71)
• transferrin receptor protein 1
• TRFR

Background

Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro. The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin.The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TFRC
• Entrez:
  • 22042(Mouse)
  • 22042(Mouse)
• Uniprot:
  • Q62351()