TfR (Transferrin R) Antibody (RI7217) [FITC]

Product Discontinued

TfR (Transferrin R) Antibody (RI7217) [FITC] Summary

• Immunogen: DMSO induced Friend erythroleukemia 745.6
• Marker: Recycling Endosome Marker
• Specificity: CD71/transferrin receptor, Mr 180-190 kDa (90-95 kDa under reducing conditions)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: TFRC
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunocytochemistry
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
  • Western Blot 1:100-1:2000

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for TfR (Transferrin R) Antibody (RI7217) [FITC]

• CD71 antigen
• CD71
• CD71TRFR
• p90
• T9
• TfR (Transferrin R)
• TfR (TransferrinR)
• TfR
• TFR1
• TFRC
• TR
• transferrin receptor (p90, CD71)
• transferrin receptor protein 1
• TRFR

Background

CD71, a type II transmembrane glycoprotein expressed on the cell surface as a homodimer, is the transferrin receptor. It is essential for the growth of normal and neoplastic cells. (1, 4) It is expressed at low levels on resting B and T lymphocytes but is upregulated during responses to antigens and mitogens, presumably reflecting the iron dependence of proliferation.4 Its expression declines with maturation and differentiation. CD71 plays a critical role in cell proliferation by controlling the supply of iron, which is essential for many metabolic pathways, through the binding and endocytosis of transferrin, the major iron carrying protein. It may also be involved in signal transduction via its association with the T cell receptor zeta chain. The monoclonal antibody RI7217 inhibits cell proliferation in vitro. (2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TFRC