TFEB Antibody

Product Discontinued

TFEB Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human TFEB. The immunogen region is: N-terminal.
• Specificity: TFEB Antibody detects endogenous levels of total TFEB protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TFEB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TFEB Antibody

• ALPHATFEB
• BHLHE35
• Class E basic helix-loop-helix protein 35
• T-cell transcription factor EB
• TCFEB
• TCFEBbHLHe35
• TFEB
• transcription factor EB

Background

Transcription factors of the basic helix-loop-helix zipper (bHLH-ZIP) family contain a basic domain, used for DNA binding, and HLH and ZIP domains, both used for oligomerization. TFEB was isolated from a human B-cell cDNA library using a binding sequence from the adenovirus major late promoter. This transcription factor probably binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TFEB Antibody (NBP1-67872)(5)

Publications using NBP1-67872

• Morishige JI, Yoshioka K, Nakata H et al. Sphingosine Kinase 1 is Involved in Triglyceride Breakdown by Maintaining Lysosomal Integrity in Brown Adipocytes Journal of lipid research 2023-09-24 [PMID: 37751791] (IHC, Mouse)
• Fernandez-Mosquera L, Diogo CV, Yambire KF et al. Acute and chronic mitochondrial respiratory chain deficiency differentially regulate lysosomal biogenesis. Sci Rep. 2017-03-27 [PMID: 28345620] (WB, Human)
• Siddiqui A, Bhaumik D, Chinta SJ et al. Mitochondrial Quality Control via the PGC1a-TFEB Signaling Pathway Is Compromised by Parkin Q311X Mutation But Independently Restored by Rapamycin. J. Neurosci. 2015-09-16 [PMID: 26377470] (ICC/IF, Mouse)
• Lee H, Lee Jk, Park Mh et al. Pathological roles of the VEGF/SphK pathway in niemann-Pick type C neurons. Nat Commun. 2014-11-24 [PMID: 25417698] (WB)
• Shibuya Y, Chang Cc, Huang Lh et al. Inhibiting ACAT1/SOAT1 in Microglia Stimulates Autophagy-Mediated Lysosomal Proteolysis and Increases AB1-42 Clearance. J. neurosci. 2014-10-22 [PMID: 25339759]

FAQs for TFEB Antibody (NBP1-67872). (Showing 1 - 2 of 2 FAQs).

1. Could you please let us know what our Customer can use as controls for the item already delivered below? TFEB Antibody (NBP1-67872) 0.1 mg
   • For nbp1-67872 we recommend mouse lung/mouse muscle cells for Western Blot and human lung carcinoma tissue for IHC.
2. Which blocking buffer should I use for your TFEB primary (NBP1-67872)? Is 5% BSA or 5% NF Milk in TBS with Tween ok?
   • 5% milk in TBS-T is what we recommend. If this gives high background, you may want to try 5% BSA.

Bioinformatics

• Gene Symbol: TFEB
• Entrez:
  • 7942(Human)
  • 21425(Mouse)
• OMIM:
  • 600744(Human)
• Uniprot:
  • P19484()