TFEB Antibody (954604) [Alexa Fluor® 647]

• Applications: WB, ICC/IF
• Clone: 954604
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

TFEB Antibody (954604) [Alexa Fluor® 647] Summary

• Immunogen: Human TFEB synthetic peptide
  Pro384-Ala446
  Accession # P19484
• Specificity: Detects human TFEB in direct ELISAs and Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Transcription Factor EB/TFEB binds E-box (5'-CANNTG-3') where it plays a role in T cell activation through expression of CD40L and CLEAR-box (5'-GTCACGTGAC-3') sequences where it promotes expression of lysosomal genes essential for regulation of autophagy.  TFEB is localized to the cytoplasm and translocates to the nucleus under aberrant lysosomal storage conditions. Phosphorylation of S211 in an MTORC1 dependent manner sequesters TFEB in the cytoplasm through association with 14-3-3 family proteins. Inhibition of MTORC1 causes dissociation from this complex and rapid accumulation in the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.