Recombinant Human TFB1M His Protein

SDS-Page: Recombinant Human TFB1M Protein [NBP1-72409] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human TFB1M His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 28-346 of Human TFB1M
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQAAKQLSQN FLLDLRLTDK IVRKAGNLTN AYVYEVGPGP GGITRSILNA DVAELLVVEK DTRFIPGLQM LSDAAPGKLR IVHGDVLTFK VEKAFSESLK RPWEDDPPNV HIIGNLPFSV STPLIIKWLE NISCRDGPFV YGRTQMTLTF QKEVAERLAA NTGSKQRSRL SVMAQYLCNV RHIFTIPGQA FVPKPEVDVG VVHFTPLIQP KIEQPFKLVE KVVQNVFQFR RKYCHRGLRM LFPEAQRLES TGRLLELADI DPTLRPRQLS ISHFKSLCDV YRKMCDEDPQ LFAYNFREEL KRRKSKNEEK EEDDAENYRL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TFB1M
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 38.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human TFB1M His Protein

• CGI75
• CGI-75
• dimethyladenosine transferase 1, mitochondrial
• EC 2.1.1
• EC 2.1.1.-
• h-mtTFB
• h-mtTFB1
• homolog of yeast mitochondrial transcription factor B
• hTFB1M
• Mitochondrial 12S rRNA dimethylase 1
• mitochondrial dimethyladenosine transferase 1
• Mitochondrial transcription factor B1
• mtTFB
• mtTFB1
• S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1
• transcription factor B1, mitochondrial

Background

TFB1M belongs to the methyltransferase superfamily. TFB1M is a dimethyltransferase that methylates mitochondrial 12S rRNA at the conserved stem loop. This protein also is part of the basal mitochondrial transcription complex and is necessary for mitochondrial gene expression. TFB1M stimulates transcription independently of the methyltransferase activity. Recombinant human TFB1M protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TFB1M