Testisin/Prss21 Antibody [Alexa Fluor™ Plus 405]

• Reactivity: Mu, Rt
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 405

Testisin/Prss21 Antibody [Alexa Fluor™ Plus 405] Summary

• Specificity: Detects recombinant mouse Testisin/Prss21 in direct ELISAs and Western blots. Detects mouse and rat Testisin/Prss21 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Prss8 and rmPrss27 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Testisin/Prss21 Antibody [Alexa Fluor™ Plus 405]

• EC 3.4.21
• Eosinophil serine protease 1
• ESP1EC 3.4.21.-
• ESP-1serine protease from eosinophils
• protease, serine, 21 (testisin)
• Prss21
• Serine protease 21
• TEST1
• TEST1testisin
• Testisin

Background

Testisin is a serine protease encoded by the PRSS21 gene. Testisin is similar in structure to tryptases, and is also known as Tryptase‑4 (1). It is predicted to have substrate specificity similar to that of trypsin (2). Testisin is most highly expressed in testicular germ cells, but is also present in eosinophils (3). Testisin is known to be required for spermatogenesis, and deficiencies of the enzyme can result in infertility (4, 5). Testisin is anchored to the cell surface through a C-terminal glycosylphosphatidylinositol anchor (6). Recombinant mouse Testisin is expressed with a truncated C-terminus, resulting in its secretion. The recombinant protein was purified as the zymogen and can undergo autoactivation under the appropriate conditions. Over aa 28-298, mouse Testisin shares 92% aa identity with rat Testisin.

1. Wong, G.W. et al. (2001) J. Biol. Chem. 276:20648.
2. Hooper, J.D. et al. (2000) Biochim. Biophys. Acta 1492:63.
3. Inoue, M. et al. (1998) Biochem. Biophys.  Res. Comm. 252:307.
4. Netzel-Arnett, S. et al. (2009) Biol. Reprod. 81:921.
5. Kawano, N. et al. (2010) Biol. Reprod. 83:359.
6. Honda, A. et al. (2002) J. Biol. Chem. 277:16976.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.