Testican 3/SPOCK3 Antibody (330404) [Alexa Fluor® 350]

• Reactivity: Mu
• Applications: WB, IP
• Clone: 330404
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 350

Testican 3/SPOCK3 Antibody (330404) [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Testican 3/SPOCK3
  Val26-Ile436
  Accession # Q8BKV0
• Specificity: Detects mouse Testican 3/SPOCK3 in direct ELISAs and Western blots. In Western blots, approximately 30% cross-reactivity with recombinant human (rh) Testican 1 and rhTestican 3 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Testican 3/SPOCK3 Antibody (330404) [Alexa Fluor® 350]

• CWCV, and Kazal-like domains proteoglycan 3
• HSAJ1454
• sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3
• SPOCK3
• TES-3
• Testican 3
• TICN3

Background

Testican 3, encoded by the SPOCK3 gene, is a proteoglycan expressed in brain (1). Human and mouse Testican 3 share 90% amino acid sequence identity, indicating a conserved function (2, 3). Testican 3 contains Ca2+-binding domain and the C-terminal acidic domain with putative glycosaminoglycan attachment sites. In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. The N-terminal region, which is unique to Testicans, is responsible for the inhibition of Testican 3 towards MMP-14/MT1-MMP activation of MMP-2 (1). The thyropin domain and the follistatin-like domain with a six cysteine Kazal-like motif may inhibit cysteine and serine proteases, respectively (4). A splice variant designated as N-Tes contains the N-terminal unique region, the follistatin-like domain and the Ca2+-binding domain, but lacks the C-terminal thyropin domain and the acidic domain (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8BKV0()