Testican 3/SPOCK3 Antibody (330403)

• Reactivity: Mu
• Applications: ELISA(Cap)
• Clone: 330403
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Testican 3/SPOCK3 Antibody (330403) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Testican 3/SPOCK3
  Val26-Ile436
  Accession # Q8BKV0
• Specificity: Detects mouse Testican 3/SPOCK3 in ELISAs. In sandwich immunoassays, less than 0.25% cross-reactivity with recombinant human (rh) Testican 1 is observed and no cross-reactivity with rhTestican 2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: SPOCK3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Mouse Testican 3/SPOCK3 Biotinylated Affinity Purified PAb (Catalog number BAF2346) Standard: Recombinant Mouse Testican 3/SPOCK3 (Catalog number 2346-PI)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Testican 3/SPOCK3 Antibody (330403)

• CWCV, and Kazal-like domains proteoglycan 3
• HSAJ1454
• sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3
• SPOCK3
• TES-3
• Testican 3
• TICN3

Background

Testican 3 encoded by the SPOCK3 gene is a proteoglycan expressed in brain (1). Human and mouse Testican 3 share 90% amino acid sequence identity, indicating a conserved function (2, 3). Testican 3 contains Ca 2+ -binding domain and the C-terminal acidic domain with putative glycosaminoglycan attachment sites. In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. The N-terminal region, which is unique to Testicans, is responsible for the inhibition of Testican 3 towards MMP-14/MT1-MMP activation of MMP-2 (1). The thyropin domain and the follistatin-like domain with a six cysteine Kazal-like motif may inhibit cysteine and serine proteases, respectively (4). A spliced variant designated as N-Tes contains the N-terminal unique region, the follistatin-like domain and the Ca 2+ -binding domain, but lacks the C-terminal thyropin domain and the acidic domain (1).

1. Nakada, M. et al. (2001) Cancer Res. 61:8896.
2. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
3. Okazaki, Y. et al. (2002) Nature 420:563.
4. Alliel, P.M. et al. (1993) Eur. J. Biochem. 214:347.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPOCK3
• Uniprot:
  • Q8BKV0()