TDRD3 Antibody

Western Blot: TDRD3 Antibody [NBP1-46821] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TDRD3 antibody NBP1-46821 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. TDRD3 was also immunoprecipitated by rabbit anti-TDRD3 antibody NBP1-46820, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Immunohistochemistry-Paraffin: TDRD3 Antibody [NBP1-46821] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TDRD3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Rt, Bv, Ch, Xp
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

TDRD3 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 601 and 651 of human tudor domain containing 3 using the numbering given in entry NP_110421.1 (GeneID 81550).
• Predicted Species: Rat (100%), Chicken (100%), Xenopus (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TDRD3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500- 1:2000
  • Immunohistochemistry-Paraffin 1:500- 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TDRD3 Antibody

• FLJ21007
• tudor domain containing 3
• tudor domain-containing protein 3

Background

TDRD3 may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation oftranslation of target mRNAs. Binds Arg/Gly-rich motifs in dimethylarginine-containing proteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TDRD3