TDG Antibody (1511) - Azide and BSA Free

Western Blot: TDG Antibody (1511) [NBP2-43718] - Analysis of 293T mock transfected (-) or transfected with a construct expressing human TDG. 10 % SDS-PAGE TDG antibody dilution: 1:10000.

Immunohistochemistry-Paraffin: TDG Antibody (1511) [NBP2-43718] - TDG antibody detects TDG protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. TDG stained by TDG antibody diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: TDG Antibody (1511) [NBP2-43718] - TDG antibody detects TDG protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat intestine. TDG stained by TDG antibody diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: TDG Antibody (1511) [NBP2-43718] - TDG antibody detects TDG protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. TDG stained by TDG antibody diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC
• Clone: 1511
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TDG Antibody (1511) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TDG Antibody (1511) - Azide and BSA Free (NBP2-43718) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TDG. The exact sequence is proprietary.
• Predicted Species: Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TDG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (88%), Rat (84%),

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for TDG Antibody (1511) - Azide and BSA Free

• EC 3.2.2.29
• TDG
• thymine-DNA glycosylaseG/T mismatch-specific thymine DNA glycosylase

Background

Thymine DNA glycosylase is encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TDG