TDAG8/GPR65 Antibody (976128) [Unconjugated]

Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human TDAG8/GPR65. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TDAG8/GPR65 Monoclonal Antibody (Catalog # MAB10077) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for TDAG8/GPR65 at approximately 85-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TDAG8/GPR65 was detected in immersion fixed TDAG8/GPR65-transfected HEK293 human embryonic kidney cell line (left panel, positive stain) and non-transfected HEK293 human embryonic kidney cell line (right panel, negative stain) using Mouse Anti-Human TDAG8/GPR65 Monoclonal Antibody (Catalog # MAB10077) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 976128
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TDAG8/GPR65 Antibody (976128) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human TDAG8/GPR65
  Met1-Glu337
  Accession # Q8IYL9
• Specificity: Detects human TDAG8/GPR65 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TDAG8/GPR65 Antibody (976128) [Unconjugated]

• G protein-coupled receptor 65
• GPR65
• G-protein coupled receptor 65
• Psychosine Receptor
• T-cell death-associated gene 8 protein
• TDAG8
• TDAG8hTDAG8

Background

Human G Protein-Coupled Receptor 65 (GPR65), also known as T-Cell Death-Associated Gene 8 Protein (TDAG8) and Psychosine Receptor, is a 337 aminoacids proton-sensing GPCR primarily expressed in lymphoid tissues and is uniquely expressed in Th17 cells; mice deficient in GPR65 are resistant to autoimmunity. GPR65 senses pH by protonation of histidine residues on its extracellular domain. Many pH-sensing G protein-coupled receptors have emerged as potential therapeutic agents in conditions that involve the response to acidotic stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8IYL9(Human)