TCTN1 Antibody - BSA Free

Western Blot: TCTN1 Antibody [NBP1-77182] - Mouse kidney tissue lysate with TCTN1 antibody at (A) 1 and (B) 2ug/mL.

Immunohistochemistry-Paraffin: TCTN1 Antibody [NBP1-77182] - Human kidney tissue with TCTN1 antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: TCTN1 Antibody - BSA Free [NBP1-77182] - Immunofluorescence of TCTN1 in Human Kidney cells with TCTN1 antibody at 20 u/mL.

Immunohistochemistry: TCTN1 Antibody - BSA Free [NBP1-77182] - Immunohistochemistry of TCTN1 in human kidney tissue with TCTN1 antibody at 5 u/mL.

Immunocytochemistry/ Immunofluorescence: TCTN1 Antibody - BSA Free [NBP1-77182] - Immunofluorescence of TCTN1 in Human Kidney cells with TCTN1 antibody at 20 u/mL.

Immunohistochemistry: TCTN1 Antibody - BSA Free [NBP1-77182] - Immunohistochemistry of TCTN1 in human kidney tissue with TCTN1 antibody at 5 u/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TCTN1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TCTN1 Antibody - BSA Free (NBP1-77182) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human TCTN1. The immunogen is located within amino acids 30 - 80 of TCTN1. Amino Acid Squence: PSTRPPGTPRAPGPS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TCTN1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  TCTN1 Peptide (NBP1-77182PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TCTN1 Antibody - BSA Free

• JBTS13
• TCTN1
• TECT1
• TECT1FLJ21127
• tectonic 1
• tectonic family member 1
• Tectonic-1

Background

TCTN1 is the founding member of the Tectonic protein family, a group of evolutionarily conserved secreted and transmembrane proteins that regulate the Hedgehog (Hh)-mediated patterning of the neural tube. During embryonic development, TCTN1 is expressed in regions that participate in Hh signaling, beginning in the gastrulation stages in the ventral node. Mice expressing mutant TCTN1 die between E13.5 and E16.5 and display holoprosencephaly, a defect associated with reduced Hh signaling, indicating the role of TCTN1 as an Hh activator. At later stages in development, TCTN1 is thought to also act as a repressor on the Hh pathway in the anterior and posterior neural tube.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TCTN1
• Uniprot:
  • NP_001076007(Human)