TCTN3 Antibody - BSA Free

Western Blot: TCTN3 Antibody [NBP1-77183] - HeLa cell lysate with TCTN3 antibody at (A) 1 and (B) 2ug/mL.

Immunocytochemistry/ Immunofluorescence: TCTN3 Antibody - BSA Free [NBP1-77183] - Immunofluorescence of TCTN3 in HeLa cells with TCTN3 antibody at 20 u/mL.

Immunocytochemistry/ Immunofluorescence: TCTN3 Antibody - BSA Free [NBP1-77183] - Immunocytochemistry of TCTN3 in HeLa cells with TCTN3 antibody at 4 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TCTN3 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TCTN3. The immunogen is located within amino acids 540 - 590 of TCTN3. Amino Acid Squence: TQKPQPPRGQPKMDWKW
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TCTN3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 4 ug/ml
  • Immunohistochemistry-Paraffin 4 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  TCTN3 Peptide (NBP1-77183PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TCTN3 Antibody - BSA Free

• C10orf61
• chromosome 10 open reading frame 61
• DKFZP564D116
• TECT3DKFZp564D116
• tectonic 3
• tectonic family member 3
• tectonic-3

Background

TCTN3 is a member of the Tectonic protein family, a group of evolutionarily conserved secreted and transmembrane proteins that regulate the Hedgehog (Hh)-mediated patterning of the neural tube. While the expression pattern and function of TCTN3 is not known, it is 58% similar to TCTN1. TCTN1 is expressed during embryonic development in regions that participate in Hh signaling, beginning in the gastrulation stages in the ventral node. Mice expressing mutant TCTN1 die between E13.5 and E16.5 and display holoprosencephaly, a defect associated with reduced Hh signaling, indicating the role of TCTN1 as an Hh activator. At later stages in development, TCTN1 is thought to also act as a repressor on the Hh pathway in the anterior and posterior neural tube.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TCTN3
• Uniprot:
  • NP_056446(Human)