TCP1-beta Antibody (5B5C4)

Western Blot: TCP1-beta Antibody (5B5C4) [NBP2-37553] - Western blot analysis using CCT2 mouse mAb against Hela (1), MCF-7 (2), Jurkat (3), T47D (4), K562 (5), A431 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.

Immunocytochemistry/Immunofluorescence: TCP1-beta Antibody (5B5C4) [NBP2-37553] - Immunofluorescence analysis of 3T3-L1 cells using CCT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow Cytometry: TCP1-beta Antibody (5B5C4) [NBP2-37553] - Flow cytometric analysis of NIH/3T3 cells using CCT2 mouse mAb (blue) and negative control (red).

Western Blot: TCP1-beta Antibody (5B5C4) [NBP2-37553] - Western blot analysis using CCT2 mAb against human CCT2 (AA: 87-290) recombinant protein. (Expected MW is 47.9 kDa)

ELISA: TCP1-beta Antibody (5B5C4) [NBP2-37553] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

TCP1-beta Antibody (5B5C4) Summary

• Immunogen: Purified recombinant fragment of human CCT2 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCT2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for TCP1-beta Antibody (5B5C4)

• Cctb
• CCT-beta
• CCTBPRO1633,99D8.1
• chaperonin containing t-complex polypeptide 1, beta subunit
• chaperonin containing t-complex polypeptide 1, subunit 2
• chaperonin containing TCP1, subunit 2 (beta)
• MGC142074
• MGC142076
• T-complex protein 1 subunit beta
• T-complex protein 1, beta subunit
• TCP-1-beta

Background

TCP1-beta encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of the gene described in this record have been observed but have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCT2