Recombinant Human TCEA1 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human TCEA1 Protein Summary

Description
A recombinant protein with a His-tag corresponding to amino acids 1 to 301 of Human TCEA1

Source: E. coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMEDEVV RFAKKMDKMV QKKNAAGALD LLKELKNIPM TLELLQSTRI GMSVNAIRKQ STDEEVTSLA KSLIKSWKKL LDGPSTEKDL DEKKKEPAIT SQNSPEAREE STSSGNVSNR KDETNARDTY VSSFPRAPST SDSVRLKCRE MLAAALRTGD DYIAIGADEE ELGSQIEEAI YQEIRNTDMK YKNRVRSRIS NLKDAKNPNL RKNVLCGNIP PDLFARMTAE EMASDELKEM RKNLTKEAIR EHQMAKTGGT QTDLFTCGKC KKKNCTYTQV QTRSADEPMT TFVVCNECGN RWKFC
Preparation
Method
E.coli
Protein/Peptide Type
Recombinant Protein
Gene
TCEA1
Purity
>90%, by SDS-PAGE

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer, pH7.5, 20% glycerol, 1mM DTT, 50mM NaCl
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 36.5kDa (325aa), confirmed by MALDI-TOF
Theoretical MW
36.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Alternate Names for Recombinant Human TCEA1 Protein

  • GTF2Stranscription elongation factor A protein 1
  • SII
  • TF2STCEA
  • TFIISTranscription elongation factor TFIIS.o
  • transcription elongation factor A (SII), 1
  • Transcription elongation factor S-II protein 1

Background

TCEA1 (Transcription elongation factor A protein 1) belongs to the TFS-II family. TCEA1 binds to Pol II and functions to cleave the nascent transcript, thereby unlocking the complex and allowing transcription to continue. Localized to the nucleus, TCEA1 contains three independently-folding domains, all of which are necessary for proper binding to Pol II. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Recombinant human TCEA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol TCEA1

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