TAFA2/FAM19A2 Antibody (447815)

• Reactivity: Hu
• Applications: WB
• Clone: 447815
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TAFA2/FAM19A2 Antibody (447815) Summary

• Immunogen: E. coli-derived recombinant human TAFA2/FAM19A2
  Ala31-His131
  Accession # AAH28403
• Specificity: Detects human TAFA2/FAM19A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human TAFA1, 3, 4, or 5 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FAM19A2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TAFA2/FAM19A2 Antibody (447815)

• Chemokine-like protein TAFA-2
• DKFZp761E1217
• DKFZp781P0552
• FAM19A2
• family with sequence similarity 19 (chemokine (C-C motif)-like), member A2
• MGC42403
• TAFA2
• TAFA-2

Background

TAFA2, also called FAM19A2, is a secreted, 11 kDa member of the FAM19/TAFA family of chemokine-like proteins (1). It is synthesized as a 131 amino acid (aa) precursor that contains a 30 aa signal sequence and a 101 aa mature chain . Like other members of the FAM19/TAFA family, with the exception of TAFA5, mature TAFA1 contains 10 regularly spaced cysteine residues that follow the pattern CX₇CCX₁₃CXCX₁₄CX₁₁CX₄CX₅CX₁₀C, where C represents a conserved cysteine residue and X represents any noncysteine amino acid (1). Human TAFA2 shares 97% aa sequence identity with mouse TAFA2 (1). TAFA2 expression can be detected in the central nervous system (CNS), colon, heart, lung, spleen, kidney, and thymus, but its expression in the CNS is 50- to 1000-fold higher than in other tissues (1). Within the CNS, TAFA2 expression is highest in the occipital and frontal cortex (3- to 10-fold more abundantly expressed than in other cortical regions) and medulla (1). Hypotheses for the biological functions of TAFA family members include modulating immune responses in the CNS by functioning as brain-specific chemokines, acting with other chemokines to optimize the recruitment and activity of immune cells in the CNS (1). Alternately, TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells (1 - 2). Finally, TAFAs may control axonal sprouting following brain injury (1).

1. Tang, Y.T. et al. (2004) Genomics 83:727.
2. Benveniste, E. (1998) Cytokine Growth Factor Rev. 9:259.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TAFA2/FAM19A2 Antibody (MAB4179)(1)

Publication using MAB4179

• A Jafari, A Isa, L Chen, N Ditzel, W Zaher, L Harkness, HE Johnsen, BM Abdallah, C Clausen, M Kassem TAFA2 induces skeletal (stromal) stem cell migration through activation of Rac1-p38 signaling Stem Cells, 2018-12-17;0(0):. 2018-12-17 [PMID: 30485583] (IHC, Human)

Bioinformatics

• Gene Symbol: FAM19A2
• Uniprot:
  • AAH28403(Human)