NAALADase-like 2/NAALADL2 Antibody [Unconjugated]

LnCAP human prostate cancer cell line was stained with Goat Anti-Human NAALADase‑like 2/NAALADL2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4665, filled histogram) or control antibody (Catalog #AB-108-C, open histogram), followed by Phycoe­rythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

• Reactivity: Hu
• Applications: WB, Flow, IP, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

NAALADase-like 2/NAALADL2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human NAALADase-like 2/NAALADL2
  Ser152-Asn795 (Met194Thr)
  Accession # CAE75743
• Specificity: Detects human NAALADase-like 2/NAALADL2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
  5% cross‑reactivity with recombinant mouse NAALADase 2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NAALADL2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunoprecipitation 25 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NAALADase-like 2/NAALADL2 Antibody [Unconjugated]

• inactive N-acetylated-alpha-linked acidic dipeptidase-like protein 2
• NAALADase L2
• NAALADase like 2
• NAALADase-like 2
• NAALADL2
• N-acetylated alpha-linked acidic dipeptidase 2
• N-acetylated alpha-linked acidic dipeptidase-like 2

Background

NAALADL2 (N-acetylated alpha -linked acidic dipeptidase like 2) is a member of the peptidase M28 family of enzymes. It is presumably a type II transmembrane (TM) protein that may have O-glycosyl hydrolase activity. Human NAALADL2 is 795 amino acids (aa) in length. It is believed to contain a cytoplasmic and TM segment at the N-terminus, followed by a peptidase domain (aa 444‑596) and a TfR-like dimerization region (aa 688‑777). There are multiple splice forms. One shows a 4 aa substitution for aa 292‑795, a second shows an alternate start site at Met18 with a 24 aa substitution for aa 314‑795, and a third contains an alternate start site at Met283, accompanied by a deletion of aa 363‑411 and 633‑795. Over aa 152‑795, human NAADADL2 is 87% and 82% aa identical to mouse and canine NAADADL2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAALADL2
• Uniprot:
  • CAE75743()