TAF6 Antibody

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Immunoprecipitation: TAF6 Antibody [NB100-60639] - Detection of Human TAF6 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

Order Details

TAF6 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 575 and 625 of human TBP-associated factor 6 using the numbering given in entry NP_005632.1 (GeneID 6878).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TAF6
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2 - 5 ug/mg lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TAF6 Antibody

  • DKFZp781E21155
  • MGC:8964
  • RNA polymerase II, E, 70/85kD
  • TAF(II)70
  • TAF(II)80
  • TAF2ERNA polymerase II TBP-associated factor subunit E
  • TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa
  • TAFII70TAFII-80
  • TAFII80TAFII-70
  • TAFII85
  • Transcription initiation factor TFIID 70 kDa subunit
  • Transcription initiation factor TFIID 80 kDa subunit
  • transcription initiation factor TFIID subunit 6

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TAF6
Entrez
Uniprot