TADA3L Overexpression Lysate (Native)

Western Blot: TADA3L Overexpression Lysate (Adult Normal) [NBL1-16679] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TADA3L.

• Reactivity: Hu
• Applications: WB

TADA3L Overexpression Lysate (Native) Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC201082 and based on accession number NM_133480. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens transcriptional adaptor 3 (NGG1 homolog, yeast)-like (TADA3L), transcript variant 2, mRNA.
• Gene: TADA3

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
• Theoretical MW: 41.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for TADA3L

• Type: Overexpression
• Protein State: Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TADA3L Overexpression Lysate (Native)

• ADA3ADA3 homolog
• FLJ20221
• FLJ21329
• hADA3ADA3-like protein
• NGG1
• STAF54
• TADA3Ltranscriptional adaptor 3 (NGG1 homolog, yeast)-like
• transcriptional adapter 3
• Transcriptional adapter 3-like
• transcriptional adaptor 3

Background

Many DNA-binding transcriptional activator proteins enhance the initiation rate of RNA polymerase II-mediated gene transcription by interacting functionally with the general transcription machinery bound at the basal promoter. Adaptor proteins are usually required for this activation, possibly to acetylate and destabilize nucleosomes, thereby relieving chromatin constraints at the promoter. The protein encoded by this gene is a transcriptional activator adaptor and has been found to be part of the PCAF histone acetylase complex. In addition, it associates with the tumor suppressor protein p53 and is required for full activity of p53 and p53-mediated apoptosis. At least four alternatively spliced variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq]. Transcript Variant: This variant (2) fails to utilize a 3' coding exon splice site and therefore has a different 3' end than variant 1. As a result, the isoform (b) encoded by variant 2 is truncated by 63 aa as compared to isoform a encoded by variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: TADA3