SYT7 Antibody (S275-14) - BSA Free

Western Blot: SYT7 Antibody (S275-14) [NBP2-22420] - Western Blot analysis of Rat brain lysates showing detection of SYT7 protein using Mouse Anti-SYT7 Monoclonal Antibody, Clone S275-14 (NBP2-22420). Primary Antibody: Mouse Anti-SYT7 Monoclonal Antibody (NBP2-22420) at 1:100, 1:250, 1:500, and 1:1000.

Immunocytochemistry/Immunofluorescence: SYT7 Antibody (S275-14) [NBP2-22420] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Synaptotagmin-7 Monoclonal Antibody, Clone S275-14 (NBP2-22420). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Synaptotagmin-7 Monoclonal Antibody (NBP2-22420) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Synaptotagmin-7 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: SYT7 Antibody (S275-14) [NBP2-22420] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-7 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Nucleus. Magnification: 60X.

Immunocytochemistry/Immunofluorescence: SYT7 Antibody (S275-14) [NBP2-22420] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Synaptotagmin-7 Monoclonal Antibody, Clone S275-14 (NBP2-22420). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-7 Monoclonal Antibody (NBP2-22420) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Synaptotagmin-7 Antibody. (D) Composite.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S275-14
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SYT7 Antibody (S275-14) - BSA Free Summary

• Description: Novus Biologicals Mouse SYT7 Antibody (S275-14) - BSA Free (NBP2-22420) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Fusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7
• Localization: Cytoplasmic Vesicle
• Specificity: Detects approx 45kDa. Does not cross-react with Synaptotagmin-6 (or others). Can identify other isoforms bands at approx 65kD.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SYT7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of Synaptotagmin-7 Antibody was sufficient for detection of Synaptotagmin-7 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for SYT7 Antibody (S275-14) - BSA Free

• IPCA-7synaptotagmin 7
• MGC150517
• prostate cancer associated protein 7
• Prostate cancer-associated protein 7
• synaptotagmin VIIPCANAP7
• synaptotagmin-7
• sytVII
• SYT-VII

Background

Synaptotagmins, such as SYT7, are brain-specific calcium-dependent phospholipid-binding proteins that play a role in synaptic exocytosis and neurotransmitter release. See MIM 600782.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYT7
• Uniprot:
  • Q9R0N7(Mouse)