Syntaxin 3 Antibody (JE40-96)

Western Blot: Syntaxin 3 Antibody (JE40-96) [NBP2-76872] - Western blot analysis of Syntaxin 3 on SH-SY-5Y cell (1) and human kidney tissue (2) lysate using anti-Syntaxin 3 antibody at 1/1,000 dilution.

Immunohistochemistry: Syntaxin 3 Antibody (JE40-96) [NBP2-76872] - Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Syntaxin 3 antibody. Counter stained with hematoxylin.

Flow Cytometry: Syntaxin 3 Antibody (JE40-96) [NBP2-76872] - Flow cytometric analysis of 293T cells with Syntaxin 3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry: Syntaxin 3 Antibody (JE40-96) [NBP2-76872] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Syntaxin 3 antibody. Counter stained with hematoxylin.

Immunohistochemistry: Syntaxin 3 Antibody (JE40-96) [NBP2-76872] - Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Syntaxin 3 antibody. Counter stained with hematoxylin.

Immunohistochemistry: Syntaxin 3 Antibody (JE40-96) [NBP2-76872] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Syntaxin 3 antibody. Counter stained with hematoxylin.

Product Discontinued

Syntaxin 3 Antibody (JE40-96) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Syntaxin 3 aa 92-218 / 289. (SwissProt: Q13277 Human; SwissProt: Q64704 Mouse; SwissProt: Q08849 Rat)
• Localization: Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: STX3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Syntaxin 3 Antibody (JE40-96)

• FLJ30906
• STX3Asyntaxin 3A
• syntaxin 3
• syntaxin-3

Background

Syntaxin 3 is a member of the SNARE family of proteins and is related to syntaxin 1. While often co-expressed with syntaxins 2 and 4 within the same cell type, its membrane localization is usually different, for instance in epithelial cells or in the exocrine pancreas. Like syntaxins 1,2, and 4 it appears to be involved in the fusion of transport vesicles with the plasma membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STX3