Recombinant Human Syntaxin 1A Protein

SDS-Page: Recombinant Human Syntaxin 1A Protein [NBC1-18338] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human Syntaxin 1A Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-226 of Human Syntaxin 1A
  
  

  Source: E.coli

  Amino Acid Sequence: MKDRTQELRT AKDSDDDDDV AVTVDRDRFM DEFFEQVEEI RGFIDKIAEN VEEVKRKHSA ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI EQEEGLNRSS ADLRIRKTQH STLSRKFVEV MSEYNATQSD YRERCKGRIQ RQLEITGRTT TSEELEDMLE SGNPAIFASG IIMDSSISKQ ALSEIETRHS EIIKLENSIR ELHDMFMDMA MLVESQ

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: STX1A
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 26.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 7.5), 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Syntaxin 1A Protein

• Brain Syntaxin
• HPC-1
• Neuron-specific antigen HPC-1
• p35-1
• STX1A
• STX1P35-1
• SYN1A
• syntaxin 1A (brain)
• Syntaxin 1A
• syntaxin-1A

Background

Syntaxin is membrane integrated Q-SNARE protein participating in exocytosis. Syntaxin is composed of an N-terminal regulatory domain(Habc), a SNARE domain(known as H3), and a single C-terminal transmembrane domain. The SNARE(H3) domain binds to both synaptobrevin and SNAP-25 forming the core SNARE complex. Recombinant syntaxin 1A protein(1-226aa) contains N-terminal domain(Habc) and t_SNARE domain(H3 domain) and this protein was overexpressed in E.coli and purified by using the conventional column chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STX1A