Recombinant Human Syntaxin 1A Protein

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SDS-Page: Recombinant Human Syntaxin 1A Protein [NBC1-18338] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Syntaxin 1A Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids 1-226 of Human Syntaxin 1A

Source: E.coli

Amino Acid Sequence: MKDRTQELRT AKDSDDDDDV AVTVDRDRFM DEFFEQVEEI RGFIDKIAEN VEEVKRKHSA ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI EQEEGLNRSS ADLRIRKTQH STLSRKFVEV MSEYNATQSD YRERCKGRIQ RQLEITGRTT TSEELEDMLE SGNPAIFASG IIMDSSISKQ ALSEIETRHS EIIKLENSIR ELHDMFMDMA MLVESQ

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
STX1A
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • SDS-Page
  • Western Blot 1:100-1:2000
Theoretical MW
26.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 7.5), 1 mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human Syntaxin 1A Protein

  • Brain Syntaxin
  • HPC-1
  • Neuron-specific antigen HPC-1
  • p35-1
  • STX1A
  • STX1P35-1
  • SYN1A
  • syntaxin 1A (brain)
  • Syntaxin 1A
  • syntaxin-1A

Background

Syntaxin is membrane integrated Q-SNARE protein participating in exocytosis. Syntaxin is composed of an N-terminal regulatory domain(Habc), a SNARE domain(known as H3), and a single C-terminal transmembrane domain. The SNARE(H3) domain binds to both synaptobrevin and SNAP-25 forming the core SNARE complex. Recombinant syntaxin 1A protein(1-226aa) contains N-terminal domain(Habc) and t_SNARE domain(H3 domain) and this protein was overexpressed in E.coli and purified by using the conventional column chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol STX1A