SVEP1 Antibody (987420) [Unconjugated]

Western blot shows lysates of MG-63 human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SVEP1 Monoclonal Antibody (Catalog # MAB97741) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SVEP1 at approximately 460 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

• Reactivity: Hu
• Applications: WB
• Clone: 987420
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SVEP1 Antibody (987420) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing human SVEP1 Peptide
  Accession # Q4LDE5
• Specificity: Detects human SVEP1 in direct Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SVEP1 Antibody (987420) [Unconjugated]

• C9orf13
• CCP module-containing protein 22
• CCP22
• FLJ16013
• FLJ90719
• POLYDOM
• selectin-like protein
• SELOB
• SEL-OB
• sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
• sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1
• SVEP1

Background

Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1 (also known as Polydom), is a 3571 aminoacids (aa), 390KDa extracellular matrix protein encoded by the SVEP1 gene. SVEP1 has been identified as a multidomain cell adhesion protein that is a ligand for integrin alpha 9 beta 1. SVEP1 has been proposed to play a crucial role in epidermis differentiation and lymphatic remodeling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q4LDE5()