SVEP1 Antibody (982903) [Unconjugated]

SVEP1 was detected in immersion fixed MG-63 human osteosarcoma cell line using Mouse Anti-Human SVEP1 Monoclonal Antibody (Catalog # MAB9774) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SVEP1 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human SVEP1 Monoclonal Antibody (Catalog # MAB9774) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in syncytiotrophoblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clone: 982903
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SVEP1 Antibody (982903) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing human SVEP1 peptide
• Specificity: Detects human SVEP1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SVEP1 Antibody (982903) [Unconjugated]

• C9orf13
• CCP module-containing protein 22
• CCP22
• FLJ16013
• FLJ90719
• POLYDOM
• selectin-like protein
• SELOB
• SEL-OB
• sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
• sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1
• SVEP1

Background

Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1 (also known as Polydom), is a 3571 aminoacids (aa), 390KDa extracellular matrix protein encoded by the SVEP1 gene. SVEP1 has been identified as a multidomain cell adhesion protein that is a ligand for integrin alpha 9 beta 1. SVEP1 has been proposed to play a crucial role in epidermis differentiation and lymphatic remodeling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.