SUZ12 Antibody (3D10) - BSA Free

Western Blot: SUZ12 Antibody (3D10) [NBP1-51687] - Western blot analysis using SUZ12 mAb against HEK293 (1) and SUZ12-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: SUZ12 Antibody (3D10) [NBP1-51687] - Immunohistochemical analysis of paraffin-embedded kidney convoluted tubule tissues (left) and esophageal cancer tissues (right) using SUZ12 mouse mAb with DAB staining.

Product Discontinued

SUZ12 Antibody (3D10) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human SUZ12 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SUZ12
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 83.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for SUZ12 Antibody (3D10) - BSA Free

• ChET 9 protein
• CHET9
• CHET9polycomb protein SUZ12
• Chromatin precipitated E2F target 9 protein
• JJAZ1
• JJAZ1Joined to JAZF1 protein
• KIAA0160Suppressor of zeste 12 protein homolog
• suppressor of zeste 12 homolog (Drosophila)
• SUZ12

Background

SUZ12 is a polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SUZ12