Substance P Overexpression Lysate

Product Discontinued

Substance P Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC205313 and based on accession number NM_003182. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens tachykinin, precursor 1 (TAC1), transcript variant beta, mRNA.
• Gene: TAC1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 12.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Substance P Overexpression Lysate

• neurokinin 1
• neurokinin 2
• neurokinin A
• Neurokinin-1
• neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma)
• neuropeptide gamma
• neuropeptide K
• NK2
• NKA
• NKNAneurokinin alpha
• NPK
• PPT
• protachykinin-1
• substance K
• Substance P
• TAC1
• TAC2neuromedin L
• tachykinin 2
• tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2
• tachykinin, precursor 1

Background

This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: Transcript variant beta encodes the full-length form of this gene. It encodes hormones substance P, neurokinin A, as well as the neuropeptide K. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: TAC1