Substance P ELISA Kit (Colorimetric)

ELISA: Substance P ELISA Kit [Alkaline Phosphatase] [KA0302] - Standard curve

Product Discontinued

Substance P ELISA Kit (Colorimetric) Summary

• Description: Quality control test: Standard curve. Substance P ELISA Kit is a competitive immunoassay for the quantitative determination of Substance P in biological fluids.
• Standard Curve Range: 9.76 to 10000 pg/mL
• Sensitivity: 8.04 pg/mL
• Assay Type: Competitive ELISA
• Inter-Assay: CV% < 6.26%
• Intra-Assay: CV% < 5.50%
• Spike Recovery: Cell Culture Supernatant: 81.00%, Plasma: 109.30%, Serum: 97.70%, Saliva: 108.30%, Urine: 105.80%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TAC1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification
• Application Notes: Useful for quantification.

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Substance P ELISA Kit (Colorimetric)

• neurokinin 1
• neurokinin 2
• neurokinin A
• Neurokinin-1
• neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma)
• neuropeptide gamma
• neuropeptide K
• NK2
• NKA
• NKNAneurokinin alpha
• NPK
• PPT
• protachykinin-1
• substance K
• Substance P
• TAC1
• TAC2neuromedin L
• tachykinin 2
• tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2
• tachykinin, precursor 1

Background

The tachykinins belong to an evolutionary conserved family of peptide neurotransmitters that share the c-terminal sequence Phe-X-Gly-Leu-Met-NH2 and have an established role in neurotransmission. The mammalian tachykinins include substance P, neurokinin A (NKA) and neurokinin B (NKB) which exert their effects by binding to specific receptors. Tachykinin peptides are important in the mediation of many physiological and pathological processes including inflammation, pain, migraine headache and allergy induced asthma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Substance P ELISA Kit (KA0302)(1)

Publication using KA0302

• Newberry K, Wang S, Hoque N et al. Development of a spontaneously active dorsal root ganglia assay using multiwell multielectrode arrays. J Neurophysiol 2016-06-01 [PMID: 27052585]

FAQs for Substance P ELISA Kit (KA0302). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TAC1