STIM2 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: STIM2 Antibody [NBP2-44170] - Staining of human Kidney tissue:

Immunohistochemistry of Sheep STIM2 Antibody. Tissue: Kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: STIM2 antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase sheep secondary antibody at 1:10,000 for 45 min at RT. Staining: STIM2 as precipitated red signal with hematoxylin purple nuclear counterstain.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Format: Azide and BSA Free

STIM2 Antibody - Azide and BSA Free Summary

• Description: This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: STIM2 Antibody was prepared from whole sheep serum produced by repeated immunizations with a synthetic peptide near the C-terminus of human STIM2. (Uniprot: Q9P246)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: STIM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1ug/ml
• Application Notes: This product is tested for ELISA and immunohistochemistry and suitable for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~84kDa corresponding to the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with mouse and human based on 100% sequence homology. Cross-reactivity with Stim II from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for STIM2 Antibody - Azide and BSA Free

• FLJ39527
• KIAA1482
• stromal interaction molecule 2

Background

STIM2 is a member of the stromal interaction molecule (STIM) family and likely arose, with related family member STIM1, from a common ancestral gene. It encodes a type 1 transmembrane protein whose function is currently unknown, but is a likely adhesion molecule with a role in early hematopoiesis. Alternative translation initiation from an AUG and a non-AUG (UUG) start site results in the production of two different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STIM2
• Uniprot:
  • Q9P246(Human)