ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (396012) [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clone: 396012
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (396012) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ST6 Gal Sialytransferase 1/ST6GAL1
  Lys27-Cys406
  Accession # P15907
• Specificity: Detects human ST6 Gal Sialytransferase 1/ST6GAL1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ST6GAL2, rhST6GALNAC1, or rhST6GALNAC5 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ST6GAL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (396012) [Unconjugated]

• Alpha 2,6-ST
• B-cell antigen CD75
• beta-galactoside alpha-2,6-sialyltransferase 1
• CD75
• CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase
• CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
• EC 2.4.99.1
• MGC48859
• sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase)
• sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase)
• Sialyltransferase 1
• SIAT1
• SIAT1Alpha 2,6-ST 1
• ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
• ST6Gal I
• ST6GAL1
• ST6GalI
• ST6N

Background

ST6GAL1 ( beta -galactoside alpha -2-6 sialyl transferase 1; also alpha -2,6-ST) is a 47-49 kDa member of the glycosyltransferase 29 family of proteins. It is principally expressed in hepatocytes, placenta and skeletal muscle, and may be unique in its ability to generate Sia-alpha -2, 6-Gal structures on liver proteins. Human ST6GAL1 is a 406 amino acid (aa) type II transmembrane protein that resides in Golgi membranes. It contains a short cytoplasmic segment (aa 1-9) and an extended luminal domain
(aa 27‑406) that possesses a phosphorylation site at Tyr369. ST6GAL1 undergoes BACE1-mediated cleavage to generate a 364 aa, 52-53 kDa soluble form. This acts within the Golgi lumen to glycosylate proteins destined for secretion. There is one potential alternative start site at Met232. Over aa 27‑406, human ST6GAL1 shares 75% aa identity with mouse ST6GAL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (MAB5924)(1)

Publication using MAB5924

• NTB Nguyen, J Lin, SJ Tay, Mariati, J Yeo, T Nguyen-Khu, Y Yang Multiplexed engineering glycosyltransferase genes in CHO cells via targeted integration for producing antibodies with diverse complex-type N-glycans Scientific Reports, 2021-06-21;11(1):12969. 2021-06-21 [PMID: 34155258] (Western Blot, Escherichia coli)

Bioinformatics

• Gene Symbol: ST6GAL1
• Uniprot:
  • P15907()