ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody - BSA Free

K562 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 antibody [N2C3] (NBP1-32451) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 antibody [N2C3] detects ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 protein by immunohistochemical analysis.Sample: Paraffin-embedded human cerebellum.ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 stained by ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 antibody [N2C3] (NBP1-32451) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Bv, Ch, Pm, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody - BSA Free (NBP1-32451) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2. The exact sequence is proprietary.
• Localization: Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein, Secreted
• Predicted Species: Mouse (96%), Rat (96%), Chicken (92%), Rhesus Macaque (99%), Chimpanzee (99%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ST3GAL2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody - BSA Free

• Alpha 2,3-ST 2
• Beta-galactoside alpha-2,3-sialyltransferase 2
• beta-galactoside alpha-2,3-sialytransferase
• CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2
• EC 2.4.99.4
• EC 2.4.99.7
• Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase
• Gal-NAc6S
• sialyltransferase 4B (beta-galactosidase alpha-2,3-sialytransferase)
• Sialyltransferase 4B
• SIAT4B
• SIAT4-B
• ST3 beta-galactoside alpha-2,3-sialyltransferase 2
• ST3GAL2
• ST3GalA.2
• ST3GalA.2ST3Gal II
• ST3GALII

Background

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4A. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ST3GAL2